Chromotek gfp-trap agarose

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August undefined, 2024

WebGet more from your GFP/RFP fusion. Alpaca GFP-Trap® is the top selling GFP antibody as it allows for one-step pulldowns that can be directly used in downstream applicaitons … WebSep 16, 2024 · ChromoTek GFP-Trap® magnetic agarose affinity beads ( see Note 2 ). 2. Low protein binding microcentrifuge tubes (e.g., LoBind® tubes). 3. Magnetic separation rack (should fit 1.5-ml microcentrifuge tubes). 4. Table-top centrifuge (cooled, with rotors suitable for 1.5- and 2-ml microcentrifuge tubes, RCF:19,000 × g ). 5.

Anti Gfp Antibody Trapped Agarose Beads ChromoTek Bioz

http://alpaca.chromotek.com/ WebThe ChromoTek GFP-Trap™ Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. £198.00 - £4914.00 Specifications View More Specs Products 5 Description Specifications Description Comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads Specifications greene county ohio november 2021 ballot

GFP-Trap® Agarose Proteintech - ptglab

WebGFP-Trap® Magnetic Agarose Kit Product code: gtmak-20 Contact [email protected] ChromoTek GmbH Am Klopferspitz 19 82152 Planegg … WebGFP-PFKFB3 was overexpressed in HEK293T cells and immunoprecipitated using GFP-Trap agarose beads. The PFKFB3 complex was then incubated with the indicated amount of recombinant human Src protein for 10 min. Western blot was used to detect tyrosine-phosphorylation level. WebProduct Information. GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads. Description. … greene county ohio obituary search

Using Chromotek GFP-Trap Magnetic Agarose beads …

ChromoBlog Immunoprecipitation - ptglab

WebChromotek GFP-Trap is supposed to be superior beads. So if anyone can think as in how to promote the binding of the complex (GFP-SG1) of +HS samples to GFP-Trap, it would be very helpful. WebYou can use Chromotek GFP-trap magnetic agarose beads for the pull-down/co-immunoprecipitation assays. These magnetic beads are coupled to GFP nanobody and are ready to use. Only you need to label ... fluffy chihuahua puppies for saleWebApr 11, 2024 · Sir2:eGFP was immunoprecipitated with ChromoTek GFP-Trap® Magnetic Agarose (ChromoTek, Planegg, Germany). Elution was performed by boiling the samples in LaemmLi buffer supplemented 200 mM DTT and proteins precipitated by addition of 4 volumes 1:1 methanol:acetone. Protein pellets were resuspended in urea 6 M, 50 mM … fluffy chicken cedar hill

"WebChromoTek GmbH To understand the biology of proteins and with that cell function in health and disease, it is crucial to be able to visualize proteins in situ and to analyze their protein-protein... " - Chromotek gfp-trap agarose

Chromotek gfp-trap agarose

GFP-Trap Agarose ChromoTek GmbH gta-20 product information

WebBoth the agarose and magnetic bead formats are extremely efficient for immunoprecipitation of GFP tagged proteins, and the usual heavy and … WebChromoTek GmbH. F2H®-Kit Basic: Kit comprises F2H®-BHK Cell Line, 1 vial 5 X 106 frozen cells (f2h-bhk); 100 µg Platform Reagent (f2h-p100); and 30 µg Control-DNA (f2h-c30) Platform Reagent: Transfection supplement enabling assembly of the nuclear PPI-platform to anchor GFP-tagged proteins; for 125 transfections (24-well format) or 500 ...

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WebOct 19, 2024 · While anti-V5-tag antibodies for conventional IP are available from multiple suppliers, ChromoTek is the first to offer anti-V5 Nanobodies bound to agarose beads for immunoprecipitation. V5-Trap is available as agarose or magnetic agarose. Higher purity of V5-Trap vs. conventional IP No contamination with heavy or light chains using V5-Trap. WebApr 11, 2024 · A 500 μl fraction of each cleared sample was mixed with 50 μl of pre-equilibrated GFP-Trap magnetic agarose (ChromoTek Cat No. gtma) or DYKDDDDK Fab-Trap Agarose (ChromoTek Cat No. ffa) and incubated over-night at 4°C rotating end-over-end. The beads were then washed twice with Wash buffer (10 mM Tris–HCl pH 7.5; 150 …

WebDec 16, 2024 · ChromoTek's Nano-Traps are optimized for the immunoprecipitation (IP) of proteins and their interacting factors. Nano-Traps comprise of a Nanobody/ V H H conjugated to beads. For 3 Nano-Traps, e.g. GFP-Trap ® , RFP-Trap ®, and Spot-Trap ®, we offer different types of beads: Agarose beads Magnetic Agarose beads Magnetic … WebAlpaca GFP-Trap® is the top selling GFP antibody as it allows for one-step pulldowns that can be directly used in downstream applicaitons such as enzyme activity assays or mass spectrometry. 2. Best results, qualitative and quantitative GFP-/RFP trap are so easier to use than any other pull down reagent.

WebJun 23, 2024 · Enhanced snoMEN Vectors Facilitate Establishment of GFP-HIF-1α Protein Replacement Human Cell Lines. PLoS ONE. 2016;11:e0154759 pubmed publisher …

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WebJun 14, 2024 · This protocol describes the immunoprecipitation with GFP-Trap® Agarose (product code: gta). GFP-Trap® Magnetic Agarose (product code: gtma) or GFP-Trap® … greene county ohio offender searchWebMay 25, 2024 · ChromoTek GFP-Trap ® is optimized for the immunoprecipitation of GFP-tagged proteins and their interacting factors. The GFP-Trap consists of the ChromoTek anti-GFP Nanobody/ V H H … fluffy chicksWebThe ChromoTek GFP-Trap™ Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. Brand: Proteintech GTMA-400 Additional Details : Weight : 0.00480kg View more versions of this product Product Code. 17353363 55828.28 NOK / 400 reactions Quantity Estimated Shipment: 12-05-2024 Add to basket Description Specifications greene county ohio nursing homesWebJan 10, 2024 · The mixture was then incubated with the GFP-Trap Agarose beads (Chromotek, pre-washed by 1 mL extraction buffer) for 4 h at 4 °C. Then, the beads were collected by spinning and washed with the extraction buffer for 3–5 times to eliminate nonspecific bindings. The bound proteins on the GFP-trap were boiled and assayed by … greene county ohio obituaries and deathsWebApr 10, 2024 · GFP-trap agarose beads (gta) were from ChromoTek. Cell lines and culture Human embryonic kidney (HEK) cell lines HEK293T (#CRL-3216) and HEK293E (#CRL-10852) were from ATCC, and MEFs were provided ... greene county ohio ombudsmanWebNano-Trap（ナノトラップ） ... Agarose (6) Alexa Fluor® 488 (1) Alexa Fluor® 568 (1) ... ChromoTek GFP recombinant antibody, VHH-mouse IgG1 Fc fusion 1 Publications; Enlarge Image ×. HeLa cells transiently expressing Mannosidase II-EGFP were immunostained with GFP recombinant antibody, VHH-mouse IgG1 Fc fusion [CTK0201] … fluffy chicken dumplings recipeWebProteintech ChromoTek GFP-Trap™ Agarose GFP-Trap™ Agarose is an affinity resin for IP of GFP-fusion proteins. Brand: Proteintech GTA-20 Additional Details : Weight : … fluffy chickens