Cisplatin a549

WebOur results identified cisplatin resistant subpopulations of NSCLC cells with a putative stem-like signature, providing a further understanding of the cellular events associated with the cisplatin resistance phenotype in lung cancer. WebApr 24, 2024 · Cell and animal experiments confirmed the effect of WDHD1 and MAPRE2 on cisplatin sensitivity in LUAD. Clinical data evaluated the impact of WDHD1 expression level on cisplatin sensitivity. Quantitative analysis of the global proteome revealed ubiquitin-dependent protein catabolism to be more active in A549/DDP cells than in A549 cells.

Cisplatin resistance in non-small cell lung cancer cells is …

WebNov 29, 2024 · Cisplatin-resistant A549 non-small cell lung cancer cells can be identified by increased mitochondrial mass and are sensitive to pemetrexed treatment Yanyun Gao, … WebThe addition of TAS reduced the IC 50 value of cisplatin in A549 and H1299 cells, indicating that TAS can significantly increase the sensitivity of lung cancer cells to cisplatin. Additionally, to determine the effects of the combination of TAS and cisplatin on the apoptosis of NSCLC cells, flow cytometry was used to detect the apoptosis rate ... rayymen technologies private limited https://vape-tronics.com

IC 50 values for cisplatin, curcuminoid, and combination …

WebAug 24, 2024 · We have next established xenograft model in nude mice via injecting LLC, A549, or H1299 cells, which stably overexpress or silence GSDME. Contrast to normal control cells, GSDME-overexpressed... WebJun 12, 2024 · Based on a published set of sequencing data, we find that hsa_circ_0001946 is a circRNA molecule with a significantly different expression level in three cell lines (human normal lung fibroblasts cell line MRC-5, human NSCLC cell line A549, cisplatin-resistant cell line A549/DDP), NSCLC tissues and paired adjacent normal tissues. WebCisplatin (DDP) has been a significant tumor chemotherapy against various solid malignant tumors, including lung adenocarcinoma, since it was approved by the Food and Drug … simply vera vera wang purse

Cisplatin-resistant A549 non-small cell lung cancer cells …

Category:Enhanced antitumor effect of cisplatin in human NSCLC cells by …

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Cisplatin a549

Hypoxia-induced PVT1 promotes lung cancer chemoresistance to …

WebNov 19, 2013 · A549 and DU145 cells were exposed to cisplatin at an IC50 dose and ROS levels measured in Annexin V-negative subpopulation as described in Materials and Methods and in Figure S2. Antimycin A was used as … WebEffect of MiR-195-5p on the Sensitivity of A549 and A549/DDP Cells to Cisplatin Sensitivity to cisplatin in the different groups was measured as the IC 50 concentration after …

Cisplatin a549

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WebApr 8, 2024 · A549 cells were seeded at a density of 3×10 4 cells/well for the C group (sensitive cells) and 9×10 4 cells/well for the CIS population (resistant cells) in 96-well …

WebJan 20, 2024 · The A549/DDP cells were plated at a density of 5 × 10 3 cells per well in a 96-well plate and cultured overnight. Then, the cells were transfected 24 h prior to glutamine deprivation with siRNA-targeting SDHB (stB0007812C, sequence 5’→3’: CCCGAAGGTTGACACCAA) or non-targeting control (RiboBio Technology) using … WebNov 1, 2024 · A549 Cells Aged Animals Antineoplastic Agents, Immunological / administration & dosage* Antineoplastic Agents, Immunological / pharmacology B7-H1 Antigen / genetics* B7-H1 Antigen / metabolism* Carcinoma, Non-Small-Cell Lung / drug therapy* Carcinoma, Non-Small-Cell Lung / genetics Carcinoma, Non-Small-Cell Lung / …

Webined if clusterin expression affects the cisplatin sensitivity in vivo; A549, A549/sCLU, and A549/pcDNA3.1 cells were injected subcutaneously into the right flank of nude mice. Cisplatin could significantly inhibit the tumor growth in mice injected with A549/pCDNA3.1 scramble cells and A549 cells compared to the mice injected with WebOct 1, 2024 · Cisplatin-resistant A549 cells (A549/CisR) were derived from A549 cells by continuous cisplatin treatment. The original parental A549 cells were initially treated …

WebApoptosis of Cisplatin-induced A549 cells was determined by immunofluorescence and flow cytometry, respectively. Western blot was performed to detect protein levels of Prx V, Bcl …

WebMay 11, 2012 · As shown in Figures 2a and b, apoptosis were induced in both A549 and H460 cells when they were treated with cisplatin alone (10 and 12.45%, respectively), … rayyes group ltdWebFeb 28, 2024 · All the new complexes showed cytotoxicity in vitro against the three cancer cell lines, but only complex 1a showed statistically significant different activity compared with cisplatin, with significantly lower IC 50 values, 4.31, 6.23, and 4.80 μM, compared with 9.71, 14.48, and 11.59 μM for cisplatin against A549, SKOV3, and MCF-7 ... simply vera vera wang purses on ebayWebIn addition, GA reduced the MRP2 and LRP protein expression level in A549/DDP cells. GA inhibits the proliferation, induces cell cycle arrest and apoptosis in A549/DDP cells. Combination of GA with cisplatin enhances the antitumor effects on cisplatin-resistant lung cancer cells by downregulating MRP2 and LRP expression. ray y martha asesinosWebAug 15, 2005 · Both BSO and siRNA sensitized melphalan and cisplatin to a small extent . In A549 cells, the GSH level was reduced to 43 ± 2% of the control in the presence of 10 μmol/L of BSO. Therefore, GSH-mediated detoxification seems to play various roles with all the drugs tested in this study, except for l-alanosine. The geldanamycin analogues ... simply vera vera wang shoesWebJan 12, 2024 · To evaluate whether cilastatin influences the antitumor effects of cisplatin in human cancer cells, SCID mice were injected with A549 human lung adenocarcinoma cells. The mice were treated with... rayynor silva wife and sanathWebFeb 22, 2024 · Lung cancer is the leading cause of cancer-related death in the Unites States, and approximately 85% of all lung cancers are classified as non-small cell lung cancer (NSCLC), which is extremely difficult to treat and its survival rate is low. After decades of clinical trials, the most effective treatments are still those that implement the … ray young derby countyWebWe used the lung cancer cell line, A549, to determine the effects of the curcuminoids and cisplatin on cell viability and several apoptotic signaling mechanisms. Cellular viability … ray younger obituary