Expand long template polymerase mix
WebExpand Long Template PCR System is a special enzyme mix that con- tains thermostable Taq DNA polymerase and Tgo DNA polymerase (1), a thermostable DNA polymerase …
Expand long template polymerase mix
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WebThe Expand™ Long Template PCR System (Product No. ELONG-RO) is an enzyme mix that contains thermostable Taq DNA polymerase and a thermostable DNA polymerase … WebExpand Long Template DNA polymerase mix (Cat. No. 11681834001; Roche, Basel, Switzerland), which is mostly Taq DNA polymerase. Zuo and Rabie developed a similar method with Taq DNA polymerase alone, and reported similarly modest cloning efficiencies (8). Taq DNA polymerase is relatively processive (60% that of Phusion ),
WebSeveral diagnostic strategies have been applied to the detection of FMR1 gene repeat expansions in fragile X syndrome. Here, we report a novel polymerase chain reaction-based strategy using the Expand Long Template PCR System (Roche Diagnostics, Mannheim, Germany) and the osmolyte betaine. WebMay 20, 2024 · Expand Long Template PCR System (Roche, cat. no. 11759060001) ... DNA polymerase mix (Expand Long Template PCR System) 0.75. 3.75 U. DNA template. x μl. 100–200 ng. Nuclease-free water.
Web• Use the Roche Expand Long Template PCR systerm (1 681 842) in the -20 enzyme freezer, small white plastic boxes. Keep the DNA polymerase mix cold. • Not included in the mix is dNTPs. Our standard stock is 2mM and the final concentration will be 0.35mM. Keep them cold. • Use0.5µl plasmid DNA per reaction. • Make at least 4 x 50µl reactions. WebApr 3, 2024 · It also produced 35× more colonies than Expand Long Template DNA polymerase mix (Cat. No. 11681834001; Roche, Basel, Switzerland), which is mostly Taq DNA polymerase. Zuo and Rabie …
http://web.as.uky.edu/biology/faculty/mirabito/BIO%20510%20Fall%202407/Expand%20Long%20Template.pdf
WebThe Expand Long Range dNTPack is optimized to efficiently amplify large genomic DNA fragments from 5kb to 25kb in combination with a threefold higher fidelity than Taq DNA … top nfl running backs of all timeWebThe Expand Long Range dNTPack is the second-generation version of the Expand Long Template PCR System, optimized and designed to consistently amplify PCR products from 5kb up to 25kb from genomic DNA (or up to 40kb from λ DNA templates). The supplied buffer can be used for all PCR product sizes. In addition, a buffer without MgCl 2, and ... top nfl runs of all timeWebIn Vitro Transcription of Long Templates (> 0.3 kb): This protocol may be used for any large-scale transcription reaction. Yields of ... 20X HMW Mix 2 µl T7 RNA Polymerase (500 units/µl) 2 µl Total reaction volume 40 µl. 6 3. Incubate at 42°C for 2–4 hrs. 4. For analysis of transcription run 1 µl of transcription reaction in a 1% pine low bookcaseWebDec 6, 2016 · Judit Pénzes. I am working on the amplification of a 3000-bp-long fragment from a viral genome wich has an average GC content of 45%. I have tryed several … top nfl rush defense 2022Web): Raise the reaction temperatures so Taq polymerase extends the primers, synthesizing new strands of DNA. This cycle repeats 25 25 - 35 35 times in a typical PCR reaction, which generally takes 2 2 - 4 4 hours, depending on the length of the DNA region being copied. top nfl running backs in historyWebExpand Long Template PCR System is a unique enzyme mix that con-tains thermostable Taq DNA polymerase and Tgo DNA polymerase (1), a thermostable DNA polymerase … pine mall heachamWebEnzyme mix GHS Classification Not a hazardous substance or mixture. Hazardous ingredients Chemical name CAS-No. Concentration (% w/w) glycerol 56-81-5 >= 50 - < 70 Taq DNA Polymerase 123340-12-5 >= 1 - < 5 Expand Long Template buffer 1, 10x conc. GHS Classification Not a hazardous substance or mixture. Hazardous ingredients top nfl running backs this season