WebHiTrap™ IMAC HP, 1 ml and 5 ml ... removed by desalting on a HiTrap Desalting, a PD-10 Desalting Column, or HiPrep™ 26/10 Desalting, (see Table 4). 28-4046-23 AE 9 Elution of bound proteins with ammonium chloride or histidine has also been reported. Leakage of Ni 2+ and Co from IMAC Sepharose WebHiTrap™ MabSelect SuRe™ 1 mL and 5 mL HiTrap MabSelect SuRe is a ready-to-use column, prepacked with MabSelect SuRe, an alkali-tolerant protein A-derived resin for capturing antibodies. This prepacked column is well suited for preparative purification of monoclonal antibodies when cleaning of the resin is of importance between the ...
HiPrep 26/10 Desalting - Cytiva
WebHiTrap Desalting are ready-to-use 5 mL columns prepacked with Sephadex G-25 Superfine resin for fast and convenient desalting and buffer exchange. Change Product. … WebHiTrap Chelating HP 1 ml and 5 ml are packed with 1 ml and 5 ml of Chelating Sepharose™ High Performance, respectively. Chelating Sepharose High Performance … top car insurance in 91773
Performing a Separation with Sephadex® - Sigma-Aldrich
WebWe recommend to use a flow rate of 0.5 mL/min for HiTrap rProtein A FF 1 mL column and 2.5 mL/min for HiTrap rProtein A FF 5 mL column. 1 Prepare collection tubes by adding 60 to 200 µL of 1 M Tris-HCl, pH 9.0 per mL of fraction to be collected. 2 Remove the stopper from the inlet and the snap-off end at the column outlet. Web17-0851-01 PD-10 2.5 ml 3.5 ml Prepacked with For desalting and buffer Sephadex™ G-25. exchange of protein Requires only extracts (M r >5000). gravity to run. 17-1408-01 HiTrap 0.1– 1.3– Prepacked with For desalting and buffer Desalting 1.5 ml 4.0 x Sephadex G-25 exchange of protein applied Superfine. extracts (M r >5000). volume … WebHisTrapHP, 1 ml and 5 ml Instructions for Use HisTrap™ HP is a ready to use HiTrap™ column, prepacked with precharged Ni Sepharose™ High Performance . This … top car insurance in 91522